Publications from Sara von Arnolds group

Full list of publications since 2000

Vestman, D., Larsson, E., Uddenberg, D., Cairney, J., Clapham, D. and von Arnold, S. 2010. Important processes during differentiation and early development of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genetic and Genomes DOI 10.1007/s11295-010-0336-4.

Clapham, D., Demel, P., Elfstrand, M., Koop, H-U., Sabala, I. and von Arnold, S. 2000. Effective biolistic gene transfer to embryogenic cultures of Picea abies and the production of transgenic plantlets. Scand. J. For. Res. 15:151-160.

Dyachok, J., Tobin, A., Price, N. and von Arnold, S. 2000. Rhizobial Nod factors stimulate somatic embryo development in Picea abies. Plant Cell Rep. 19:290-297.

Sabala,I., Clapham, D., Elfstrand, M. and von Arnold S. 2000. Tissue-specific experssion of Pa18, a putative lipid transfer protein gene, during embryo development in Norway spruce (Picea abies). Plant Mol. Biol. 42: 461-478.

Filonova, L., Bozhkov, P. and von Arnold, S 2000. Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-laps tracking. J. Exp. Bot. 51 /343: 249-264.

Brukhin, V., Clapham, D., Elfstrand, M. and von Arnold, S. 2000. Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce. Plant Cell Rep. 19:899-903.

Mathur, G., von Arnold, S, and Nadgauda, R. 2000. Studies on somatic embryogenesis from immature zygotic embryos of chir pine (Pinus roxburghii). Current Science 79 (7) 999-1004.

Filonova, L., Bozhkov, P., Brukhin, V., Danierl, G., Zhivotovsky, B. and von Arnold, S. 2000. Developmental programmed cell death in plant embryogenesis: exploring a model system of Norway spruce somatic embryogenesis. J. Cell Sci. 113(24) 4399-4411.

Clapham DH, Ekberg I, Norell L, Vince-Prue D. 2001. Circadian timekeeping for the photoperiodic control of budset in Picea abies (Norway spruce) seedlings. Biological Rhythm Research 32: 479-487.

Vahala, T. and von Arnold, S. 2001. Two APETALA 2 like genes are differentially expressed during development. J. Exp. Bot. 52 (358) 1111-1115.

Lindroth, A., Kvarnheden, A. and von Arnold, S. 2001. Isolation of a PSTAIRE cdc2 gene from Pinus contorta and its expression in adventitious root development. Plant Physiol. Bioch. 39:107-114.

Högberg, K-A., Bozhkov, P., Grönroos, R. and von Arnold, S. 2001. Dissection of critical factors affecting ex vitro performance of somatic embryo plants of Norway spruce. Scand. J. For. Res. 16 (4) 295-304.

Lindroth, A., Saarikoski, P., Flygh, G., Clapham, D., Grönroos, R., Thelander, M., Ronne, H. and von Arnold, S. 2001. Two S-adenosylmethiononine synthetase encoding genes are differentially expressed during adventitious root development in Pinus contorta. Plant Mol. Biol. 46:335-346.

Elfstrand, M., Fossdal, C-G., Swedjemark, G., Clapham, D., Olsson, O., Sitbon, F., Sharma, P., Lönneborg, A. and von Arnold, S. 2001. Identification of candidate genes for use in molecular breeding - A case study with the Norway spruce defensin-like gene, spi1. Silvae Genetica 50 (2) 75-81.

Elfstrand, M., Fossdal, C-G., Sitbon, F., Olsson, O., Lönneborg, A. and von Arnold, S. 2001. Overexpression of the endogenous peroxidase-like gene spi2 in transgenic Norway spruce plants results in increased total peroxidase activity and reduced growth. Plant Cell Rep. 20:596-603.

Ingouff, M., Farbos, I., Lagercrantz, U. and von Arnold, S. 2001. PaHB1 is an evolutionary conserved HD-GL2 homeobox gene defining the protoderm during Norway spruce embryo development. Genesis 30:220-230.

Elfstrand, M., Sitbon, F., Lapierre, C., Bottin, A, von Arnold, S, 2002. Altered lignin structure and resistance to pathogens in spi 2-expressing tobacco plants. Planta 214:708-716.

Bozhkov, P., Filonova, L. and von Arnold, S. 2002. A key developmental switch during Norway spruce somatic embryogenesis is induced by withdrawal of growth regulators and is associated with cell death and extracellular acidification. Biotechnology and Bioengineering Vol 77 (6) 658-667.

Dyachok, J., Wiwegwe, M., Kenne, L. and von Arnold, S. 2002. Endogenous Nod-factor-like signal molecules suppress cell death and promote early somatic embryo development in Norway spruce. Plant Physiol. 128: 523-533.

Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J. and Filonova, L. 2002. Important aspects of somatic embryogenesis. (Review article) Plant Cell Tissue and Organ Culture. 69:233-249.

Ciavatta, V., Egertsdotter, U., Clapham, C., von Arnold, S. and Cairney, J. 2002. A promoter from the loblolly pine PtNIP1;1 gene directs expression in an early-embryogenesis and suspensor-specific fashion. Planta 215:694-698.

Hjortswang, H., Filonova, L., Vahala, T. and von Arnold, S. 2002. Modified expression of the Pa18 gene interferes with somatic embryo development in Norway spruce. Plant Growth Reg. 38:75-82.

Parasharami, V.A., Clapham, D., Poonawala,I.S., von Arnold, S. and Nadgauda, R.S. 2002. Gene transfer to regenerating cotyledons of Pinus roxburghii and embryos of Pinus contorta. Plant Cell Biotech. Mol. Biol. 3:43-50.

Filonova, L., von Arnold, S., Daniels, J. and Bozhkov, P. 2002. Programmed cell death eliminates all but one embryo in polyembryonic plant seed. Cell Death Diff. 9:1057-1062.

Van Zyl, L., von Arnold, S., Bozhkov, P., Chen, Y., Egertsdotter, U., MacKay, J., Sederoff, R., Shen, J., Zelena, L. and Clapham, D. 2002. Heterologous array analysis in Pinaceae: Hybridization of high density arrays of Pinus taeda cDNA from needles and embryogenic cultures of P. taeda, P. sylvestris or Picea abies. Comp. Func. Genomics 3:306-318.

Clapham DH, Ekberg I, Eriksson G, Norell L, Vince-Prue D. 2002. Requirement for far-red light to maintain secondary needle extension growth in northern but not southern populations of Pinus sylvestris (Scots pine). Physiol Plant 114: 207-212.

Hjortswang, H., Sundås, A., Bharathan, G., Bozhkov, P. von Arnold, S. and Vahala, T. 2002. KNOTTED 1-like homeobox genes of a gymnosperm, Norway spruce, expressed during somatic embryogenesis. Plant Physiol. Biochem. 40:837-843.

Högberg, K-A., Bozhkov, P. and von Arnold, S. 2003. Height growth and within-clone variation of Norway spruce clones propagated by somatic embryogenesis. Tree Physiol. 23:211-216.

Van Zyl, L., Bozhkov, P., Clapham, D., Sederoff, R. and von Arnold, S. 2003. Up, down and up again is a signature global gene expression pattern at the beginning of gymnosperm embryogenesis. Mechanism of Development, Gene Expression Patterns 3:83-91.

Whiteley RE, Black-Samuelsson, Clapham D. 2003. Development of microsatellite markers for the European white elm (Ulmus laevis Pall.) and cross-species amplification within the genus Ulmus. Mol Ecol Notes 3: 598-600.

Smertenko, A., Bozhkov, P., Filonova, L., von Arnold, S. and Hussey, P. 2003. Re-organisation of the cytoskeleton during developmental programmed cell death in Picea abies embryos. Plant Journal 33:813-824.

Ingouff, M., Farbos, M., Wiweger, M. and von Arnold, S. 2003. The molecular characterization of PaHB2, a homeobox gene of the HD-GL2 family expressed during embryo development in Norway spruce. J. Exp. Bot. 54:1343-1350.

Footitt, S., Ingouff, M., Clapham, D. and von Arnold, S. 2003. Expression of the viviparous 1(Pavp1) and p32 protein kinase (cdc2Pa) genes during somatic embryogenesis in Norway spruce (Picea abies). J. Exp. Bot. 54:1711-1719.

Wiweger, M., Farbos, I., Ingouff, M., Lagercrantz, U. and von Arnold, S. 2003. Expression of Chia4-Pa chitinase genes during somatic and zygotic embryo development in Norway spruce (Picea abies): similarities and differences between gymnosperm and angiosperm class IV chitinases. J. Exp. Bot. 54 (393) 2691-2699.

Wadenbäck, J., Clapham, D., Gellerstedt, G. and von Arnold, S. 2004. Variation in content and composition of lignin in young wood of Norway spruce. Holzforschung 58:107-115.

Bozhkov, P., Filonova, L., Suarez, M., Helmersson, A., Smertenko, P., Zhivotovsky, B. and von Arnold, S. 2004. VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation. Cell Death Diff. 11:175-182.

Suarez, M. F., Filonova, L., Smertenko, A., Savenkov, E., Clapham, C., von Arnold, S., Zhivotovsky, B. and Bozhkov, P. 2004. Metacaspase-dependent programmed cell death is essential for plant embryogenesis. Curr. Biol. 14 R339- R340.

Helmersson, A., von Arnold, S., Kornel, B. and Bozhkov, P. 2004. High stability of nuclear microsatellite loci during the early stages of somatic embryogenesis in Norway spruce. Tree Physiol. 24: 1181-1186.

Brinker, M., van Zyl, L., Liu, W., Craig, D., Sederoff, R., Clapham, D. and von Arnold, S. 2004. Microarray analyses of gene expression during adventitious root development in Pinus contorta. Plant Physoiol. 135:1526-1539.

Stasolla, C., Bozhkov, P., Chu, T-M., van Zyl, L., Egertsdotter, U., Suarez, M., Craigh, D., Wolfinger, R., von Arnold, S. and Sederoff, R. 2004. Variation in transcript abundance during somatic embryogenesis in gymnosperms. Tree Physiol. 24: 1073-1085.

Wadenbäck, J., Clapham, D., Craig, D., Sederoff, R., Peter, G., von Arnold, S. and Egertsdotter, U. 2005. Comparison of standard exponential and linear techniques to amplify small cDNA samples for microarrays. BMC Genomics 6:Art.No. 61.

Högberg, K-A., Flygh, G., Grönroos, R. and von Arnold, S. 2005. Field performance of Pinus contorta propagated vegetatively via adventitious buds. Scand. J. For. Res. 20:318-321.

Zelena, L., Sorochinsky, B., von Arnold, S., van Zyl, L, and Clapham, D. 2005. Indications of limited altered gene expression in Pinus sylvestris trees from the Chernobyl region. J. Environ. Radio. 84:363-373.

Parasharami, V., Naik, V., von Arnold, S., Nadgauda, R. and Clapham, C. 2006. Stable transformation of mature embryos and regeneration of transgenic plants of Chir pine (Pinus roxburghii). Plant Cell Rep. 24 (2) 708-714.

Shirgurkar, M., Clapham, D., von Arnold, S. and Nadgauda, R. 2006. An efficient protocol for genetic transformation and shoot regeneration of tumeric (Curcuma longa L.) via particle bombardment. Plant Cell Rep. 25 (2) 112-116.

Rodriguez, M.P.., Fernanda Suarez, M., Heredia, R., Concepcion, A., Breton, D., Trontin, J.-F., Filonova, L., von Arnold, S., Harvengt, L. and Canova, F. 2007. Developmental regulation of glutamine sythetase genes during embryogenesis of pine. New Phytol. 169:35-44.

Burg, K., Helmersson, A., Bozhkov, P. and von Arnold, S. 2007. Developmental and genetic variation in nuclear microsatellite instability during pine embryogenesis. J. Exp. Bot. 58:687-698.

Wadenbäck, J., von Arnold, S., Egertsdotter, U., Walter, M., Grima-Pettenati, J., Goffner, D., Gellerstedt, G., Gullion, T. and Clapham, D. 2007. Lignin biosynthesis in transgenic Norway spruce plants harbouring an antisense construct for cinnamoyl CoA reductase (CCR). Transgenic research DOI 10. 11248-007.

Gyllenstrand N, Clapham D, Källman T, Lagercrantz U. 2007. A Norway spruce FLOWERING LOCUS T homolog is implicated in control of growth rhythm in conifers. Plant Physiol 144: 248-257.

Larsson, E., Sitborn, F., Ljung, K. and von Arnold, S. 2008. Inhibited polar auxin transport results in aberrant embryo development in Norway spruce. New Phytol. 177:356-366.

Helmersson, A., Jansson, G. , Bozhkov, P. and von Arnold, S. 2008 Microsatellite stability of somatic embryo plants of Picea abies varies among families. Scand. J. For. Res. 23, 2-11.

Helmersson, A., von Arnold, S. and Bozhkov, P. 2008. The level of free intracellular zink mediates programmed cell death/cell survival decisions in plant embryos. Plant Physiology 147:1158-1167.

Larsson, E., Sitbon, F. and von Arnold, S. 2008 Polar auxin transport controls suspensor fate. Plant Signalling and Behavior 3:469-470.

Helmersson, A. and von Arnold, S. 2009. Embryogenic cell lines of Juniperus communis: easy establishment and embryo maturation, limited germination. Plant Cell Tissue and Organ Culture 96:211-217 .

Kien, N., Quang, T., Jansson, G., Harwood, C., Clapham, D. and von Arnold, S. 2009. Cellulose content as a selection trait in breeding for kraft pulp yield in Eucalyptus urophylla. Ann. For. Sci. 66:711-718.

Ubhayasekera, W, Rawat, R., Wing Tak Ho, S., Wiweger, M., von Arnold, S., Chey, M-L and Mowbray, S.L. 2009. The first crystal structures of a family 19 class IV chitinase: the enzyme from Picea abies. Plant Mol. Biol. 71 (3) 277-289.

Quang T., Kien N., von Arnold S., Jansson G., Thinh H. and Clapham D. 2010. Relationship of wood composition to growth traits of selected open-pollinated families of Eucalyptus urophylla from a progeny trial in Vietnam. New Forests 39 (3) 301-312.

Berlin, S.U., Lagercrantz, U., von Arnold, S., Öst, T. and Rönnberg-Wästljung A. C. 2010. High density linkage mapping and evolution of paralogs and ortologs in Salix and Populus. BMC Genomics 11: 129.

Vestman, D., Larsson, E., Uddenberg, D., Cairney, J., Clapham, D. and von Arnold, S. 2011. Important processes during differentiation and early development of somatic embryos of Norway spruce as revealed by changes in global gene expression. Tree Genetic and Genomes 7:347-362.

Uddenberg, D., Valladares, S., Abrahamsson, M., Sundström, J., Sundås-Larsson, A. and von Arnold, S. 2011. Embryogenic potential and expression of embryogenesis-related genes in conifers are affected by treatment with a histone deacetylase inhibitor. Planta 234:527-539.

Quang, T., Hallingbäck, H., Gyllenstrand, N, von Arnold, S. and Clapham, D. 2012. Expression of genes of cellulose and lignin synthesis in Eucalyptus urophylla and its relation to some economic traits. Trees – Strucure and Function. 26:893-901.

Abrahamsson, M., Valladares, S., Larsson, E., Clapham, D. and von Arnold, S. 2012. Patterning during somatic embryogenesis in Scots pine in relation to polar auxin transport and programmed cell death.  Plant Cell Tiss Organ Cult 109:391-400.

Larsson, E., Sitbon, F. and von Arnold,S. 2012. Differential regulation of Knotted1-like genes during establishment of the shoot apical meristem in Norway spruce (Picea abies). Plant Cell Rep 31:1053-1060.

Larsson, E., Sundström, J., Sitbon, F. and von Arnold, S. 2012. Expression of PaNAC01, a Picea abies CUP-SHAPED COTYLEDON orthologue, is regulated by polar auxin transport and associated with differentiation of the shoot apical meristem and formation of separated cotyledons. Annals of Botany 110:923-934.

Uddenberg, D., Reimgård, J., Clapham, D., Almqvist, C., von Arnold, S., Emanuelsson, O. and Sundström, J. 2013. Early cone-setting in Picea abies var. acrocona is associated with increased transcriptional activity of MADS-box transcription factor. Plant Physiol 161:813-823.

Quang, T.H., Gyllenstrand, N., Jansson, G., Griffin, R., von Arnold, S. and Clapham, D. 2013. Evaluating population structure by neutral makers and quantitative genetic parameters in a thinned progeny trial of Eucalyptus urophylla.   Journal of Tropical Forest Science 25 (3): 350-360.

Hedman, H., Zhu, T., von Arnold, S. and Sohlberg, J. 2013. Analysis of the WUSCHEL-RELATED HOMEOBOX gene family in the conifer Picea abies reveals extensive conservation as well as dynamic patterns. BMC Plant Biology 13:89.

Zhu, T., Moschou, P. N., Alvarez J.M., Sohlberg, J. and Von Arnold, S. 2014. WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce. Journal of Experimental Botany 65:6543-6552.

Review articles

Von Arnold, S. 2003. Transgenic gains in conifer breeding. In Forest Biotechnology in Europe. The challenge, The Promise, The Future. Eds S. MacCord and K. Gartland. 10-12.

Von Arnold, S., Bozhkov, P., Clapham, D., Dyachok, J., Filonova, L., Högberg, K-A., Ingouff, M. and Wiweger, M. 2005. Propagation of Norway spruce via somatic embryogenesis. In Liquid culture systems for in vitro plant propagation. A.K. Hvoslef-Eide and W. Preil (eds) pp283-294.

Book chapters
Clapham, D., Newton, R., Sen, S. and von Arnold, S. 2000. Transformation of Picea species. In Molecular Biology of Woody Plants. Vol 2. 105-118. Eds M Jain and SC Minocha .

Clapham D, Ekberg I, Little CHA and Savolainen O. 2001. Molecular biology of conifer frost tolerance and potential applications to tree breeding. In: Conifer Cold Hardiness (Ed. Bigras FJ, Colombo SJ) ,Kluwer Academic Publishers, Dordrecht, pp 187-219.


Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J. and Filonova, L. 2007. Background of somatic embryogenesis. In: Plant Propagation by Tissue Culture, A. Cassels and G.J. de Klerk (eds). Kluwer. pp 335-354.

Von Arnold, S. and Clapham, D. 2007. Somatic embryogenesis. Methods in Molecular Biology 427:31-47.

Eriksson G, Ekberg I and Clapham D. 2008. Introduction to Forest Genetics. 185 pp


Popular publications

von Arnold, S. 1999. Forskarutbildning till vad? En genomlysning av svensk forskarutbildning. Sveriges universitets-och högskoleförbund.

Clapham, D., Ekberg, I., Elfstrand, M., Swedjemark, G. and von Arnold, S. 2001. Genvägen i skogen – ny teknik ger värdefull kunskap om geners function. Fakta Skog Nr 4.

Von Arnold 2007. Skogsbioteknik ger mer bioenergi. I Bioenergi- till vad och hur mycket? Pp 211-221. ISBN: 978-91-540-5995-9.